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Ribozymes catalyze ligation of RNA, protein

The first ribozymes that catalyze the joining of RNA to protein have been developed by AUTHORS. The ribozymes were created using a combination of in vitro selection and rational design. The ribozymes catalyze phosphoamide bond formation between the 5 end of an RNA molecule and the amino terminus of a peptide. This catalytic activity is unique for ribozymes but is related to that of some naturally occurring enzymes such as guanyltransferase and DNA and RNA ligases, which catalyze phosphoamide bond formation in enzyme-nucleotide reaction intermediates. The development and characterization of the new ribozymes extend the repertoire of ribozyme catalysis and could permit proteins with amplifiable RNA tags to be created both in vitro and in vivo. The findings present an opportunity to apply RNA catalysis toward the affinity tagging, detection, and encoding of polypeptides, the researchers suggest.



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