Main > NEPHROLOGY > Renal Failure > Acute Renal Failure > Treatment > Peptide Growth Factors. > Co.: USA. N Program > Patent > Claims Etc > Claim 1: Regenerating Kidney Tissue > Method Comprise Steps: (a) Prepare > Purified/Isolated Autocrine Growth > Factor which Effects Growth Kidney > Epithelial Cells; Characteristics: > (1) Apparent MW=3,500-10,000 D. > based on SDS-PAGE & Ultrafiltration > (2) Activity of Growth of Kidney > Epithelial Cells Resistant to Inhib > ition by: (i) 65 mM Dithiothreitol > for > 1 Hour; (ii) Freezing for 1 > Week or (iii) Heating at 56C 30 min > .; (3) Activity of Growth of Kidney > Epithelial Cells Inhibited by: > (i) Heating at 100C 15 min.; (ii) > Exposure to 0.1% Trifluoroacetic > Acid for 1 Hour or (iii) Treat.: > 100 .mu.g/ml of Trypsin 3 Hours at > 37C. (4) Activity of Growth Kidney > Epithelial Cells Stable in 1% AcOH

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