Main > NUCLEIC ACID > Dye Labeled Nucleic Acids > Stabilization. > Patent > Claims > Claim 1: Redn. of Degradation of > Fluorescent Dye-Labeled Polymerase > Extension Products: Add Formamide > & Additional Compd. Selected: > Base; Buffer & Reducing Agent. > Claim 2: Compd.: DiThioThreitol. > Claim 3: NaHCO3. Claim 4: Dye: > Fluorescent Energy Transfer Dye > Claim 5: Dye: 5CFB-dR110-2. > Claim 7: Polymerase Extension Prodt > Terminate in 4 Different > Dideoxyribonucleotides (D) & > 1 > (D) Labeled with Dye (Claim 4). > Claim 8: Labeled (D): > ddG-EO-5CFB-dR110-2. Claim 9: > Addition of Compd. Prevents > Excessive Fluorescence at 530 nm; > Excessive Fluorescence Migrates > in PolyAcrylamide Gel Electrophores > is or Capillary Electrophoresis > Similar to Polymerase Extension > Prodt. which is about 90 bases long > Claim 10: DNA Template Sequence > Determn. Method.. Patent Assignee




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