| METHOD |
Nanoparticles tag bacteria Rapid and sensitive methods for detecting bacteria are needed to protect food and water supplies and for early disease diagnosis. Most assays for bacteria detection require DNA amplification or enrichment of the bacteria, making the methods laborious and time-consuming. Authors have developed an assay that can detect a single bacterial cell within 20 minutes using fluorescent nanoparticles conjugated to antibodies for the bacteria Each nanoparticle encapsulates thousands of fluorescent dye molecules in a silica matrix, and thousands of antibody-conjugated nanoparticles can bind to a single bacterial cell. The resulting signal is highly amplified, allowing the detection of a single cell. |
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