PATENT ASSIGNEE'S COUNTRY | USA |
UPDATE | 03.99 |
PATENT ASSIGNEE | Vanderbilt University |
PATENT CLAIMS |
Recombinant cytochrome P450 purification method - Fractionating host cells to prepare their membranes - Adding a nonionic detergent (0.8-2 %) & in detergent/pro tein = 4:1 - 10:1 - Adding ionic detergent (0.4-0.8%) & in detergent/protein = 2:1-4:1 - Centrifuging to remove insoluble materials - Purifying by: (i) through diethylaminoethyl-beaded column (ii) through carboxymethyl-beaded column & (iii) through hydroxylapatite column |
PATENT PHOTOCOPY | Available on request |
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