Main > PROTEINS > Cytochrome P450 > Purification

Product USA. V

PATENT ASSIGNEE'S COUNTRY USA
UPDATE 03.99
PATENT ASSIGNEE Vanderbilt University
PATENT CLAIMS Recombinant cytochrome P450 purification method
- Fractionating host cells to prepare their membranes
- Adding a nonionic detergent (0.8-2 %) & in detergent/pro
tein = 4:1 - 10:1
- Adding ionic detergent (0.4-0.8%) & in detergent/protein =
2:1-4:1
- Centrifuging to remove insoluble materials
- Purifying by: (i) through diethylaminoethyl-beaded column
(ii) through carboxymethyl-beaded column & (iii) through
hydroxylapatite column
PATENT PHOTOCOPY Available on request

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