| STUDY |
Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana Brett Lahner1, Jiming Gong2, Mehrzad Mahmoudian1, Ellen L Smith1, Khush B Abid2, Elizabeth E Rogers3, Mary L Guerinot4, Jeffrey F Harper5, John M Ward6, Lauren McIntyre7, Julian I Schroeder2 & David E Salt1 1. Center for Plant Environmental Stress Physiology, Horticulture Building, 625 Agriculture Mall Drive, Purdue University, West Lafayette, Indiana 47907, USA. 2. Division of Biological Science, Cell and Developmental Biology Section and Center for Molecular Genetics 0116, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA. 3. Department of Nutritional Sciences, 217B Gwynn Hall, University of Missouri, Columbia, Missouri 65211, USA. 4. Department of Biological Sciences, 6044 Gilman, Dartmouth College, Hanover, New Hampshire 03755, USA. 5. The Scripps Research Institute, Department of Cell Biology, Mail Drop BCC283, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. 6. Department of Plant Biology, University of Minnesota, 250 Biological Science Center, 1445 Gortner Avenue, St. Paul, Minnesota 55108, USA. 7. Agricultural Genomics, Department of Agronomy, 915 State Street, Purdue University, West Lafayette, Indiana 47907, USA. Correspondence should be addressed to D E Salt. e-mail: dsalt@purdue.edu Understanding the functional connections between genes, proteins, metabolites and mineral ions is one of biology's greatest challenges in the postgenomic era. We describe here the use of mineral nutrient and trace element profiling as a tool to determine the biological significance of connections between a plant's genome and its elemental profile. Using inductively coupled plasma spectroscopy, we quantified 18 elements, including essential macro- and micronutrients and various nonessential elements, in shoots of 6,000 mutagenized M2 Arabidopsis thaliana plants. We isolated 51 mutants with altered elemental profiles. One mutant contains a deletion in FRD3, a gene known to control iron-deficiency responses in A. thaliana. Based on the frequency of elemental profile mutations, we estimate 2–4% of the A. thaliana genome is involved in regulating the plant's nutrient and trace element content. These results demonstrate the utility of elemental profiling as a useful functional genomics tool. |
| UPDATE | 08.03 |
| AUTHOR | Purdue Uni.'s Salt David E. |
| LITERATURE REF. |
Nat. Biotechnol., Published online: 31 August 2003, doi:10.1038/nbt865 October 2003 Volume 21 Number 10 pp 1215 - 1221 |
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