| PATENT NUMBER | This data is not available for free |
| PATENT GRANT DATE | 31.12.02 |
| PATENT TITLE |
Tumor necrosis factor receptor-associated factors |
| PATENT ABSTRACT | The invention concerns new tumor necrosis factor receptor associated factors, designated TRAF. The new factors are capable of specific association with the intracellular domain of the type 2 TNF receptor (TNF-R2), and are involved in the mediation of TNF biological activities |
| PATENT INVENTORS | This data is not available for free |
| PATENT ASSIGNEE | This data is not available for free |
| PATENT FILE DATE | January 7, 1997 |
| PATENT REFERENCES CITED |
Pharmacia Catalog pp. 133, 142-143 (1994). Clonetech Catalog pp. 19, 192-194 (1994). Berg, J., "Zinc Fingers and Other Metal-Binding Domains" Journal of Biological Chemistry 265(12):6513-6516 (1990). Beutler, B. and Cerami, A., "Tumor necrosis, cachexia, shock, and inflammation: a common mediator" Ann. Rev. Biochem. 57:505-518 (1988). Blake et al., "The sequences of the human and mouse c-cbl proto-oncogenes show v-cbl was generated by a large truncation encompassing a proline-rich domain and a leucine zipper-like motif" Oncogene 6:653-657 (1991). Brockhaus et al., "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies" Proc. Natl. Acad. Sci. USA 87:3127-3131 (1990). Chan et al., "Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro Autoantigen" J. Clin. Invest. 87:68-76 (1991). Cheng et al., "Involvement of CRAF1, a relative of TRAF, in CD40 signaling"Science 267 (8) :1494-1498 (1995). Chevray et al., "Protein interaction cloning in yeast: identification of mammalian proteins that react with the Leucine Zipper of Jun." Proc. Natl. Acad. Sci. USA 89:5789-5793 (1992). Chien et al., "The Two-Hybrid System: A method to identify and clone genes for proteins that interact with a protein of interest" Proc. Natl. Acad. Sci. USA 88:9578-9582 (1991). Deng et al., "COP1, an arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G, homologous domain" Cell 71:791-801 (1992). Driscoll and Williams, "Two divergently transcribed genes of dictyostelium discoideum are cyclic AMP-inducible and coregulated during development" Molecular & Cellular Biology 7(12):4482-4489 (1987). Durfee et al., "The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit" Genes and Development 7(4):555-569 (1993). Engelmann et al., "Antibodies to a Soluble Form of a Tumor Necrosis Factor (TNF) Receptor Have TNF-like Activity" Journal of Biological Chemistry 265(24):14497-14504 (1990). Espevik et al., "Characterization of binding and biological effects of monoclonal antibodies against a human tumor necrosis factor receptor" Journal of Experimental Medicine 171:415-426 (1990). Fields and Song, "A novel genetic system to detect protein-protein interactions" Nature 340:245-246 (1989). Fiers, W., "Tumor necrosis factor characterization at the molecular, cellular and In Vivo level" FEBS Letters 285(2):199-212 (1991). Freemont et al., "A novel cysteine-rich sequence motif" Cell 64:483-484 (1991). Goeddel et al., "TNF receptor signal transduction" Keystone Symposium on Hematopoiesis, Breckenridge, Colorado, Jan. 4-11, 1994 (Abstract only) Supplement 0 (Supp 0(18 Pt A):A011 (1994). Goeddel et al., "Tumor Necrosis Factors: Gene Structure and Biological Activities" Cold Spring Harbor Symposia on Quantitative Biology LI:597-609 (1986). Goodwin et al., "Molecular cloning and expression of the type 1 and type 2 murine receptors for tumor necrosis factor" Molecular & Cellular Biology 11:3020-3026 (1991). Gray et al., "Cloning and Expression of cDNA for Human Lymphotoxin, a Lymphokine with Tumour Necrosis Activity" Nature 312:721-724 (1984). Haupt et al., "Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E.mu.-myc transgenic mice" Cell 65:753-763 (1991). Hohmann et al., "Expression of the types A and B tumor necrosis factor (TNF) receptors is independently regulated, and both receptors mediate activation of the transcription factor NF-kB" Journal of Biological Chemistry 265:22409-22417 (1990). Hohmann et al., "Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha)" Journal of Biological Chemistry 264(25):14927-14934 (1989). Hu et al., "A novel RING finger protein interacts with the cytoplasmic domain of CD40" Journal of Biological Chemistry 269(48):30069-30072 (1994). Inoue et al., "Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a ring finger protein" Proc. Natl. Acad. Sci. USA 90:11117-11121 (1993). Itoh et al., "Protein heterogeneity in the human Ro/SSA ribonucleoproteins" J. Clin. Invest. 87:177-186 (1991). Jones et al., "The saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence" Nucl. Acids Res. 16(14):7119-7131 (1988). Kohno et al., "A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor" Proc. Natl. Acad. Sci. USA 87:8331-8335 (1990). Landschulz et al., "The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins" Science 204:1759-1764 (1988). Lenardo and Baltimore, "NF-kB: A pleiotropic mediator of inducible and tissue-specific gene controll" Cell 58:227-229 (1989). Lewis et al., "Cloning and expression of cDNAs for two distinct murine tumor necrosis factor receptors demonstrate one receptor is species specific" Proc. Natl. Acad. Sci. USA 88:2830-2834 (1991). Loetscher et al., "Molecular Cloning and Expression of the Human 55 kd Tumor Necrosis Factor Receptor" Cell 61:351-359 (Apr. 20,1990). Mardon and Page, "The sex-determining region of the mouse Y chromosome encodes a protein with a highly acidic domain and 13 zinc fingers" Cell 56:765-770 (1989). Miller et al., "Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus Oocytes" EMBO Journal 4(6):1609-1614 (1985). Miyatake et al., "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes" EMBO Journal 4:2561-2568 (1985). Mosialos et al., "The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family" Cell 80:389-399 (1995). Naume, B. et al., "Involvement of the 55- and 75-kDa tumor necrosis factor receptors in the generation of lymphokine-activated killer cell activity and proliferation of natural killer cells" J. Immunol. 146:3035-3048 (1991). Neta et al., "Comparison of the In Vivo effects of rIL-1 and rTNF in radioprotein, induction of CSF and of acute phase reactants" Fed. Proc. (Abstract) 46(4):1200 (1987). Neta et al., "Interdependence of the radioprotective effects of human recombinant interleukin 1.alpha., tumor necrosis factor .alpha., granulocyte colony-stimulating factor, and murine recombinant granulocyte-macrophage colony-stimulating factor" J. Immunol. 140(1):108-111 (1988). Neta et al., "Interleukin 1 is a radioprotector" J. Immunol. 136(7):2483-2485 (Apr. 1, 1986). Nietfeld et al., "Second-order repeats in xenopus laevis finger proteins" J. Mol. Bil. 208:639-659 (1989). Nophar et al., "Soluble forms of tumor necrosis factor receptors (TNF-Rs). The cDNA for the type I TNF-R, cloned using amino acid sequence data of its soluble form, encodes both the cell surface and a soluble form of the receptor" EMBO Journal 9:3269-3278 (1990). Old, L.J., "Tumor Necrosis Factor" Sci. Am. 258(5):59-75 (1988). Opipari et al., "The A20 cDNA induced by tumor necrosis factor .alpha. encodes a Novel type of zinc finger protein" Journal of Biological Chemistry 265:14705-14708 (1990). Patarca et al., "rpt-1, an intracellular protein from helper/inducer T cells that regulates gene expression of interleukin 2 receptor and human immunodeficiency virus type 1" Proc. Natl. Acad. USA 85:2733-2737 (1988). Pennica et al., "Human Tumour Necrosis Factor: Precursor Structure, Expression and Homology to Lymphotoxin" Nature 312:724-729 (1984). Reddy et al., "A novel zinc finger coiled-coil domain in a family of nuclear proteins" Trends Biochem. Sci. 17:344-345 (1992). Rothe et al., "A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDA tumor necrosis factor receptor" Cell 78:681-692 (1994). Rothe, M., "TRAF1 and TRAF2" 5th International Congress on TNF and Related Cytokines, Monterey, CA (May 31, 1994) ((Oral Presentation)) (1994). Ruiz i Altaba et al., "Xfin: an embryonic gene encoding a multifingered protein in Xenopus" EMBO Journal 6(10):3065-3070 (1987). Sato et al., "A novel member of the TRAF family of putative signal transducing proteins binds to the cytosolic domain of CD40" FEBS Letters 358(2):113-118 (1995). Schall et al., "Molecular Cloning and Expression of a Receptor for Human Tumor Necrosis Factor" Cell 61:361-370 (Apr. 20, 1990). Schatz et al., "The V(D)J recombination activating gene, RAG-1" Cell 59:1035-1048 (1989). Shalaby et al., "Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors" Journal of Experimental Medicine 172:1517-1520 (1990). Smith et al., "A Receptor for Tumor Necrosis Factor Defines an Unusual Family of Cellular and Viral Proteins" Science 248:1019-1023 (May 25, 1990). Smith et al., "The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death" Cell 76:959-962 (1994). Song et al., "Association of a RING Finger Protein with the Cytoplasmic Domain of the Human Type-2 Tumor Necrosis Factor Receptor" Biochemical Journal 309:825-829 (1995). Stanley et al., "The structure and expression of the murine gene encoding granulocyte-macrophage colony stimulating factor: evidence for utilisation of alternative promoters" EMBO Journal 4:2569-2573 (1985). Takahashi et al., "Developmentally regulated expression of a human "finger"-containing gene encoded by the 5' half of the ret transforming gene" Molecular & Cellular Biology 8(4):1853-1856 (1988). Tartaglia and Goeddel, "Two TNF receptor" Immunology Today 13:151-153 (1992). Tartaglia et al., "A novel domain within the 55kd TNF receptor signals cell death" Cell 74(5):845-853 (1993). Tartaglia et al., "The two different receptors for tumor necrosis factor mediate distinct cellular responses" Proc. Natl. Acad. Sci. USA 88:9292-9296 (1991). Tomita et al., "The neurospora uvs-2 gene encodes a protein which has homology to yeast RAD18, with unique zinc finger motifs" Mol. Gen. Genet. 238:225-233 (1993). Urbaschek et al., "Tumor necrosis factor induced stimulation of granulopoiesis and radioprotein" Lymphokine Research 6(3):179-186 (1987). Vinson et al., "Scissors-grip model for DNA recognition by a family of leucine zipper proteins" Science 246:911-916 (1989). |
| PATENT PARENT CASE TEXT | This data is not available for free |
| PATENT CLAIMS |
What is claimed is: 1. An isolated human tumor necrosis factor receptor associated factor (TRAF) prepared by (a) screening a human recombinant cDNA library prepared from tissue expressing human TNF-R2 at a detectable level with one or more labeled oligonucleotide probe(s) having about 30 to 50 bases derived from the nucleotide sequence encoding marine TRAF1 (SEQ. ID. NO:1) or murine TRAF2 (SEQ. ID. NO:3), wherein said probe(s) are designed based on TRAF1 or TRAF2 regions which have the least codon redundance, under stringent conditions comprising overnight incubation at 42.degree. C. in a solution comprising 20% formamide, 5.times.SSC, 50 mM sodium phosphate (pH7.6), 5.times.Denhardt's solution, 10% dextran sulfate, and 20 g/ml denature, sheared salmon sperm DNA; (b) inserting the DNA hybridizing to said probe(s) into a replicable expression vector; (c) transforming a recombinant host cell with said expression vector; (d) culturing the transformed host cell; and (e) recovering said human TRAF. 2. The isolated human of claim 1, wherein said oligonucleotide probes are derived from the nucleotide sequence encoding murine TRAF1 (SEQ. ID. NO: 1). 3. The isolated human of claim 1, wherein said oligonucleotide probes are derived from the nucleotide sequence encoding murine TRAF2(SEQ ID. NO: 3. -------------------------------------------------------------------------------- |
| PATENT PHOTOCOPY | Available on request |
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