Main > GENOMICS > Same Gene can Code for > Variety of Proteins. depending on > How Transcribed RNA is Processed > Splice Variant Discrimination Assay > Devised by

Product USA. IU

TECHNOLOGY The same gene can code for a variety of proteins, depending on how the transcribed RNA is processed. Because the probes used in RNA microarrays are so long, they are limited in their ability to distinguish the slight differences in these so-called splice variants. In the assay, strands of complementary DNA are annealed to the mRNA sequences on both sides of a splice junction. These complementary oligonucleotides are ligated and then become templates for amplification by the polymerase chain reaction. The oligonucleotides contain sequences that guide them to specific locations on a fiber-optic microbead array, and fluorescent tags allow them to be visualized using a charge-coupled device camera. The team validated the assay using the alternative splicing of E1A transcripts, an adenovirus oncogene involved in growth and development, as a model system. They also used the assay for the multiplexed analysis of 97 mRNA sequences derived from 23 genes in human cancer cell lines
UPDATE 04.02
AUTHOR This data is not available for free
LITERATURE REF. This data is not available for free

Want more information ?
Interested in the hidden information ?
Click here and do your request.


back