| TECHNOLOGY |
"It would have every gene in it," author says. "It would be made broadly available without restriction. These genes would be expression-ready, and they would be sequenced-verified." Essentially, the FLEXGene repository would be a rack of bar-coded tubes, each containing a different gene incorporated in a master clone. Master clones are circular pieces of DNA (plasmids) that, when engineered into cells, induce those cells to express the corresponding protein. Proteins would be expressed from master clones by a rapid, automatable technique called recombinational cloning. "It happens in a tube over 45 minutes." "The only product you get back is the one you want, and the technique is essentially 100% efficient." Using master clones, researchers will be able to move genes into expression systems and then study where a protein becomes localized in cells, what other proteins it interacts with, and what its function is. They will also be able to use expressed proteins for structural genomics studies, to induce antibodies, to construct protein microarrays, or for screening with small organic molecules. Author says his group's next step "is to try and encourage the entire scientific community that this is an important enough project that we need to fund it. It's a big project--I won't kid you." To set up the FLEXGene repository will cost about $100 million, and the project should be a collaborative effort by industry and government, he says. According to author , "A repository like this is going to exist sometime in the next 10 years. The question is, How do we do it and when do we do it?" |
| UPDATE | 07.01 |
| AUTHOR | This data is not available for free |
| LITERATURE REF. | This data is not available for free |
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