| TECHNOLOGY |
He and his coworkers recently developed another labeling strategy called AQUA (an acronym for "absolute quantitation") that measures a protein's absolute, as opposed to relative, abundance. In this technique, a chemically synthesized version of a targeted protein is used as a standard to determine the absolute amount of that protein in a biological sample. Author and coworkers recently used it to obtain the absolute quantification of phosphorylated versus nonphosphorylated protein directly from total cell lysates
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