| TECHNOLOGY |
Researchers are developing gas-phase ion mobility strategies. In IMS, mixts of ions are separated in a gas as they traverse a electric field in a “drift tube” The sepn is based on differences on the ions’ charge, mass, & shape. Group uses IMS as a highly parallel way to prepare samples for analysis by time-of-flight tandem mass spectrometry (TOF-MS/MS). “Our strategy is to take whole cells digest the cell proteins to form peptides, separate the peptides by standard LC – yielding typically > 100 fractions – separate them in a drift tube by IMS (see Abbrev.), & then analyze them by reflectron time-of-flight MS/MS” The multidimensional technique is thus called LC-IMS-TOF-MS/MS. It makes it possible to carry out MS/MS experiments in parallel – that is, more quickly – whereas “up to now it’s been a serial process” This type of multidimensional sepn is analogous to lining up the residents of NYC by height, separating them further by weight, & then distinguishing among them once again by hair color. “You can separate a lot of people pretty effectively by using just these simple characteristics” The technique actually provides 4 degrees of sepn., because MS/MS is two- dimensional. “We think we have the capacity to separate 10 million peptides” |
| UPDATE | 04.02 |
| AUTHOR | This data is not available for free |
| LITERATURE REF. | This data is not available for free |
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