| PATENT NUMBER | This data is not available for free |
| PATENT GRANT DATE | 09.01.2001 |
| PATENT TITLE |
Human XAG-1 polynucleotides and polypeptides |
| PATENT ABSTRACT |
The present invention relates to huXAG-1, huXAG-2 and huXAG-3 proteins which are novel human growth factors. In particular, isolated nucleic acid molecules are provided encoding the huXAG-1, huXAG-2 and huXAG-3 proteins. huXAG-1, huXAG-2 and huXAG-3 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of huXAG-1, huXAG-2 or huXAG-3 activity. Also provided are diagnostic and therapeutic uses of huXAG-1. huXAG-2 and huXAG-3. |
| PATENT INVENTORS | This data is not available for free |
| PATENT ASSIGNEE | This data is not available for free |
| PATENT FILE DATE | August 22, 1997 |
| PATENT REFERENCES CITED |
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NCBI Entrez, GenBank Report, Accession No. AA442829, from Hillier, L., et al. (Jun. 1997). NCBI Entrez, GenBank Report, Accession No. AA469767, from Marra, M., et al. (Jun. 1997). NCBI Entrez, GenBank Report, Accession No. AA473910, from Marra, M., et al. (Jun. 1997). NCBI Entrez, GenBank Report, Accession No. AA498578, from Marra, M. et al. (Jul. 1997). NCBI Entrez, GenBank Report, Accession No. AA512381, from Marra, M., et al. (Jul. 1997). NCBI Entrez, GenBank Report, Accession No. AA509472, from Marra, M., et al. (Jul. 1997). NCBI Entrez, GenBank Report, Accession No. AA538512, from Marra, M., et al. (Jul. 1997). NCBI Entrez, GenBank Report, Accession No. AA476675, from Hillier, L., et al. (Aug. 1997). NCBI Entrez, GenBank Report, Accession No. AA533047, from National Cancer Institute, Cancer Genome Anatomy Project (Aug. 1997). NCBI Entrez, GenBank Report, Accession No. AA565444, from National Cancer Institute, Cancer Genome Anatomy Project (Sep. 1997). 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NCBI Entrez, GenBank Report, Accession No. AA583091, from National Cancer Institute, Cancer Genome Anatomy Project (Sep. 1997). NCBI Entrez, GenBank Report, Accession No. AA607599, from Marra, M., et al. (Sep. 1997). NCBI Entrez, GenBank Report, Accession No. W11310, from Marra, M., et al. (Oct. 1997). NCBI Entrez, GenBank Report, Accession No. AA425142, from Hillier, L., et al. (Oct. 1997). NCBI Entrez, GenBank Report, Accession No. AA426228, from Hillier, L., et al. (Oct. 1997). NCBI Entrez, GenBank Report, Accession No. AA622524, from National Cancer Institute, Cancer Genome Anatomy Project (Oct. 1997). NCBI Entrez, GenBank Report, Accession No. AA638538, from Marra, M., et al. (Oct. 1997). NCBI Entrez, GenBank Report, Accession No. AA632103, from National Cancer Institute, Cancer Genome Anatomy Project (Oct. 1997). NCBI Entrez, GenBank Report, Accession No. AA421562, from Hillier, L., et al. (Nov. 1997). NCBI Entrez, GenBank Report, Accession No. AA691538, from Marra, M., et al. (Dec. 1997). NCBI Entrez, GenBank Report, Accession No. AA710639, from Marra, M., et al. (Dec. 1997). NCBI Entrez, GenBank Report, Accession No. AA776960, from Hillier, L., et al. (Feb. 1998). EST fragment (Genbank Accession No. W37614, May 1996. STS fragment (Genbank Accession No. G28839, May 1996. STS fragment (Genbank Accession No. G19689, May 1996. |
| PATENT PARENT CASE TEXT | This data is not available for free |
| PATENT CLAIMS |
What is claimed is: 1. An isolated polynucleotide comprising a nucleic acid encoding at least 30 contiguous amino acids of SEQ ID NO:2, wherein said polynucleotide is not any one of SEQ ID NOs:35-39. 2. The isolated polynucleotide of claim 1, further comprising a heterologous polynucleotide. 3. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 1 into a vector. 4. A vector comprising the isolated polynucleotide of claim 1. 5. The vector of claim 4, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 6. A host cell comprising the isolated polynucleotide of claim 1. 7. The host cell of claim 6, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 8. A method of producing a polypeptide encoded by the polynucleotide of claim 1, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 9. A polypeptide produced by the method of claim 8. 10. A composition comprising the polynucleotide of claim 1. 11. The complement of the isolated polynucleotide of claim 1. 12. An isolated polynucleotide comprising a nucleic acid encoding at least 50 contiguous amino acids of SEQ ID NO:2. 13. The isolated polynucleotide of claim 12, further comprising a heterologous polynucleotide. 14. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 12 into a vector. 15. A vector comprising the isolated polynucleotide of claim 12. 16. The vector of claim 15, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 17. A host cell comprising the isolated polynucleotide of claim 12. 18. The host cell of claim 17, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 19. A method of producing a polypeptide encoded by the polynucleotide of claim 12, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 20. A polypeptide produced by the method of claim 19. 21. A composition comprising the polynucleotide of claim 12. 22. The complement of the isolated polynucleotide of claim 12. 23. An isolated polynucleotide comprising a nucleic acid encoding a fragment of SEQ ID NO:2, wherein said fragment stimulates cell proliferation as a growth factor. 24. The isolated polynucleotide of claim 23, further comprising a heterologous polynucleotide. 25. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 23 into a vector. 26. A vector comprising the isolated polynucleotide of claim 23. 27. The vector of claim 26, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 28. A host cell comprising the isolated polynucleotide of claim 23. 29. The host cell of claim 28, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 30. A method of producing a polypeptide encoded by the polynucleotide of claim 23, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 31. A polypeptide produced by the method of claim 30. 32. A composition comprising the polynucleotide of claim 23. 33. The complement of the isolated polynucleotide of claim 23. 34. An isolated polynucleotide comprising a nucleic acid encoding a fragment of the amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641, wherein said fragment stimulates cell proliferation as a growth factor. 35. The isolated polynucleotide of claim 34, further comprising a heterologous polynucleotide. 36. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 34 into a vector. 37. A vector comprising the isolated polynucleotide of claim 34. 38. The vector of claim 37, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 39. A host cell comprising the isolated polynucleotide of claim 34. 40. The host cell of claim 39, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 41. A method of producing a polypeptide encoded by the polynucleotide of claim 34, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 42. A polypeptide produced by the method of claim 41. 43. A composition comprising the polynucleotide of claim 34. 44. The complement of the isolated polynucleotide of claim 34. 45. An isolated polynucleotide comprising 30 contiguous nucleotides of the coding region of SEQ ID NO:1, wherein said polynucleotide is not any one of SEQ ID NOs:35-39. 46. The isolated polynucleotide of claim 45, comprising 50 contiguous nucleotides of the coding region of SEQ ID NO:1. 47. The isolated polynucleotide of claim 45, further comprising a heterologous polynucleotide. 48. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 45 into a vector. 49. A vector comprising the isolated polynucleotide of claim 45. 50. The vector of claim 49, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 51. A host cell comprising the isolated polynucleotide of claim 45. 52. The host cell of claim 51, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 53. A composition comprising the isolated polynucleotide of claim 45. 54. The complement of the isolated polynucleotide of claim 45. 55. An isolated nucleic acid molecule comprising a first polynucleotide which is capable of hybridizing at 42.degree. C. in 50% formamide, 5.times. SSC, 50 mM sodium phosphate, 5.times. Denhardt's solution, 10% dextran sulfate, and 20 .mu.g/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1.times. SSC at 65.degree. C., to a second polynucleotide selected from the group consisting of: (a) a polynucleotide having the sequence of the coding region of the cDNA clone contained in ATCC Deposit No. 97641 or the complementary sequence thereof; (b) a polynucleotide having the sequence of the coding region of SEQ ID NO:1 or the complementary sequence thereof, wherein said first polynucleotide is not any one of SEQ ID NOs:35-39. 56. The isolated nucleic acid molecule of claim 55, which is DNA. 57. The isolated nucleic acid molecule of claim 55, which is RNA. 58. The isolated nucleic acid molecule of claim 55, further comprising a heterologous polynucleotide. 59. A method of producing a recombinant vector comprising inserting the isolated nucleic acid molecule of claim 55 into a vector. 60. A vector comprising the isolated nucleic acid molecule of claim 55. 61. The vector of claim 60, wherein said nucleic acid molecule is operably associated with a heterologous regulatory sequence. 62. A host cell comprising the isolated nucleic acid molecule of claim 55. 63. The host cell of claim 62, wherein said isolated nucleic acid molecule is operably associated with a heterologous regulatory sequence. 64. A composition comprising the nucleic acid molecule of claim 55. 65. An isolated polynucleotide comprising a nucleic acid encoding a portion of SEQ ID NO:2, wherein said portion is selected from the group consisting of: (a) amino acids -1 to 23 of SEQ ID NO:2; (b) amino acids 41 to 52 of SEQ ID NO:2; (c) amino acids 70 to 83 of SEQ ID NO:2; (d) amino acids 93 to 105 of SEQ ID NO:2; and (e) amino acids 118 to 130 of SEQ ID NO:2. 66. The isolated polynucleotide of claim 65, wherein said portion is (a). 67. The isolated polynucleotide of claim 66, comprising nucleotides 128 to 199 of SEQ ID NO:1. 68. The isolated polynucleotide of claim 65, wherein said portion is (b). 69. The isolated polynucleotide of claim 68, comprising nucleotides 251 to 286 of SEQ ID NO:1. 70. The isolated polynucleotide of claim 65, wherein said portion is (c). 71. The isolated polynucleotide of claim 70, comprising nucleotides 338 to 379 of SEQ ID NO:1. 72. The isolated polynucleotide of claim 65, wherein said portion is (d). 73. The isolated polynucleotide of claim 72, comprising nucleotides 407 to 445 of SEQ ID NO:1. 74. The isolated polynucleotide of claim 65, wherein said portion is (e). 75. The isolated polynucleotide of claim 74, comprising nucleotides 482 to 520 of SEQ ID NO:1. 76. The isolated polynucleotide of claim 65, further comprising a heterologous polynucleotide. 77. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 65 into a vector. 78. A vector comprising the isolated polynucleotide of claim 65. 79. The vector of claim 78, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 80. A host cell comprising the isolated polynucleotide of claim 65. 81. The host cell of claim 80, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 82. A method of producing a polypeptide encoded by the polynucleotide of claim 65, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 83. A polypeptide produced by the method of claim 82. 84. A composition comprising the polynucleotide of claim 65. 85. The complement of the isolated polynucleotide of claim 65. 86. An isolated polynucleotide comprising a nucleic acid at least 95% identical to a reference nucleic acid encoding amino acids 1 to 155 of SEQ ID NO:2, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference nucleic acid and that allow gaps of up to 5% of the total number of nucleotides of said reference nucleic acid. 87. The isolated polynucleotide of claim 86, wherein said reference nucleic acid is nucleotides 131 to 595 of SEQ ID NO:1. 88. The isolated polynucleotide of claim 86, wherein said reference nucleic acid encodes amino acids -19 to 155 of SEQ ID NO:2. 89. The isolated polynucleotide of claim 88, wherein said reference nucleic acid is nucleotides 74 to 595 of SEQ ID NO:1. 90. The isolated polynucleotide of claim 88, wherein said reference nucleic acid encodes amino acids -20 to 155 of SEQ ID NO:2. 91. The isolated polynucleotide of claim 90, wherein said reference nucleic acid is nucleotides 71 to 595 of SEQ ID NO:1. 92. The isolated polynucleotide of claim 86, further comprising a heterologous polynucleotide. 93. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 86 into a vector. 94. A vector comprising the isolated polynucleotide of claim 86. 95. The vector of claim 94, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 96. A host cell comprising the isolated polynucleotide of claim 86. 97. The host cell of claim 96, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 98. A composition comprising the polynucleotide of claim 86. 99. The complement of the isolated polynucleotide of claim 86. 100. An isolated polynucleotide comprising a nucleic acid at least 95% identical to a reference nucleic acid encoding the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference nucleic acid and that allow gaps of up to 5% of the total number of nucleotides of said reference nucleic acid. 101. The isolated polynucleotide of claim 100, wherein said reference nucleic acid encodes the complete amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 102. The isolated polynucleotide of claim 100, wherein said reference nucleic acid is the coding region of the cDNA clone in ATCC Deposit No. 97641. 103. The isolated polynucleotide of claim 100, further comprising a heterologous polynucleotide. 104. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 100 into a vector. 105. A vector comprising the isolated polynucleotide of claim 100. 106. The vector of claim 105, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 107. A host cell comprising the isolated polynucleotide of claim 100. 108. The host cell of claim 107, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 109. A composition comprising the polynucleotide of claim 100. 110. The complement of the isolated polynucleotide of claim 100. 111. An isolated polynucleotide comprising a nucleic acid encoding an amino acid sequence at least 95% identical to a reference amino acid sequence consisting of amino acids 1 to 155 of SEQ ID NO:2, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference amino acid sequence and that allow gaps of up to 5% of the total number of residues of said reference amino acid sequence. 112. The isolated polynucleotide of claim 111, wherein said reference amino acid sequence consists of amino acids -19 to 155 of SEQ ID NO:2. 113. The isolated polynucleotide of claim 112, wherein said reference amino acid sequence consists of amino acids -20 to 155 of SEQ ID NO:2. 114. The isolated polynucleotide of claim 111, further comprising a heterologous polynucleotide. 115. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 111 into a vector. 116. A vector comprising the isolated polynucleotide of claim 111. 117. The vector of claim 116, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 118. A host cell comprising the isolated polynucleotide of claim 111. 119. The host cell of claim 118, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 120. A method of producing a polypeptide encoded by the polynucleotide of claim 111, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 121. A polypeptide produced by the method of claim 120. 122. A composition comprising the polynucleotide of claim 111. 123. The complement of the isolated polynucleotide of claim 111. 124. An isolated polynucleotide comprising a nucleic acid encoding an amino acid sequence at least 95% identical to a reference amino acid sequence consisting of the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference amino acid sequence and that allow gaps of up to 5% of the total number of residues of said reference amino acid sequence. 125. The isolated polynucleotide of claim 124, wherein said reference amino acid sequence consists of the complete amino acid sequence of the cDNA clone in ATCC Deposit No. 97641. 126. The isolated polynucleotide of claim 124, further comprising a heterologous polynucleotide. 127. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 124 into a vector. 128. A vector comprising the isolated polynucleotide of claim 124. 129. The vector of claim 128, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 130. A host cell comprising the isolated polynucleotide of claim 124. 131. The host cell of claim 130, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 132. A method of producing a polypeptide encoded by the polynucleotide of claim 124, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 133. A polypeptide produced by the method of claim 132. 134. A composition comprising the polynucleotide of claim 124. 135. The complement of the isolated polynucleotide of claim 124. 136. An isolated polynucleotide comprising a nucleic acid encoding amino acids 1 to 155 of SEQ ID NO:2. 137. The isolated polynucleotide of claim 136, comprising nucleotides 131 to 595 of SEQ ID NO:1. 138. The isolated polynucleotide of claim 136, comprising a nucleic acid encoding amino acids -19 to 155 of SEQ ID NO:2. 139. The isolated polynucleotide of claim 138, comprising nucleotides 74 to 595 of SEQ ID NO:1. 140. The isolated polynucleotide of claim 138, comprising a nucleic acid encoding amino acids -20 to 155 of SEQ ID NO:2. 141. The isolated polynucleotide of claim 140, comprising nucleotides 71 to 595 of SEQ ID NO:1. 142. The isolated polynucleotide of claim 136, further comprising a heterologous polynucleotide. 143. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 136 into a vector. 144. A vector comprising the isolated polynucleotide of claim 136. 145. The vector of claim 144, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 146. A host cell comprising the isolated polynucleotide of claim 136. 147. The host cell of claim 146, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 148. A method of producing a polypeptide encoded by the polynucleotide of claim 136, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 149. A polypeptide produced by the method of claim 148. 150. A composition comprising the polynucleotide of claim 136. 151. The complement of the isolated polynucleotide of claim 136. 152. An isolated polynucleotide comprising a nucleic acid encoding the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 153. The isolated polynucleotide of claim 152, comprising a nucleic acid encoding the complete amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 154. The isolated polynucleotide of claim 152, comprising the coding region of the cDNA clone in ATCC Deposit No. 97641. 155. The isolated polynucleotide of claim 152, further comprising a heterologous polynucleotide. 156. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 152 into a vector. 157. A vector comprising the isolated polynucleotide of claim 152. 158. The vector of claim 157, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 159. A host cell comprising the isolated polynucleotide of claim 152. 160. The host cell of claim 159, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 161. A method of producing a polypeptide encoded by the polynucleotide of claim 152, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 162. The polypeptide produced by the method of claim 161. 163. A composition comprising the polynucleotide of claim 152. 164. The complement of the isolated polynucleotide of claim 152. 165. An isolated polypeptide comprising a fragment of the amino acid sequence in SEQ ID NO:2, wherein said fragment stimulates cell proliferation as a growth factor. 166. The isolated polypeptide of claim 165, further comprising a heterologous polypeptide. 167. A composition comprising the polypeptide of claim 165. 168. An isolated polypeptide comprising a fragment of the amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641, wherein said fragment stimulates cell proliferation as a growth factor. 169. The isolated polypeptide of claim 168, further comprising a heterologous polypeptide. 170. A composition comprising the polypeptide of claim 168. 171. An isolated polypeptide comprising 30 contiguous amino acids of SEQ ID NO:2. 172. The isolated polypeptide of claim 171, comprising 50 contiguous amino acids of SEQ ID NO:2. 173. The isolated polypeptide of claim 171, further comprising a heterologous polypeptide. 174. A composition comprising the polypeptide of claim 171. 175. An isolated polypeptide comprising a portion of SEQ ID NO:2, wherein said portion is selected from the group consisting of: (a) amino acids -1 to 23 of SEQ ID NO:2; (b) amino acids 24 to 32 of SEQ ID NO:2; (c) amino acids 41 to 52 of SEQ ID NO:2; (d) amino acids 70 to 83 of SEQ ID NO:2; (e) amino acids 93 to 105 of SEQ ID NO:2; and (f) amino acids 118 to 130 of SEQ ID NO:2. 176. The isolated polypeptide of claim 175, wherein said portion is (a). 177. The isolated polypeptide of claim 175, wherein said portion is (b). 178. The isolated polypeptide of claim 175 wherein said portion is (c). 179. The isolated polypeptide of claim 175, wherein said portion is (d). 180. The isolated polypeptide of claim 175, wherein said portion is (e). 181. The isolated polypeptide of claim 175, wherein said portion is (f). 182. The isolated polypeptide of claim 175, further comprising a heterologous polypeptide. 183. A composition comprising the polypeptide of claim 175. 184. An isolated polypeptide comprising an amino acid sequence at least 95% identical to a reference amino acid sequence consisting of amino acids 1 to 155 of SEQ ID NO:2, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference amino acid sequence and that allow gaps of up to 5% of the total number of residues of said reference amino acid sequence. 185. The isolated polypeptide of claim 184, wherein said reference sequence consists of amino acids -19 to 155 of SEQ ID NO:2. 186. The isolated polypeptide of claim 184, wherein said reference sequence consists of amino acids -20 to 155 of SEQ ID NO:2. 187. The isolated polypeptide of claim 184, further comprising a heterologous polypeptide. 188. A composition comprising the polypeptide of claim 184. 189. An isolated polypeptide comprising an amino acid sequence at least 95% identical to a reference amino acid sequence consisting of the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference amino acid sequence and that allow gaps of up to 5% of the total number of residues of said reference amino acid sequence. 190. The isolated polypeptide of claim 189, wherein said reference amino acid sequence consists of the complete amino acid sequence of the cDNA clone in ATCC Deposit No. 97641. 191. The isolated polypeptide of claim 189, further comprising a heterologous polypeptide. 192. A composition comprising the polypeptide of claim 189. 193. An isolated polypeptide comprising amino acids 1 to 155 of SEQ ID NO:2. 194. The isolated polypeptide of claim 193, comprising amino acids -19 to 155 of SEQ ID NO:2. 195. The isolated polypeptide of claim 194, comprising amino acids -20 to 155 of SEQ ID NO:2. 196. The isolated polypeptide of claim 193, further comprising a heterologous polypeptide. 197. A composition comprising the polypeptide of claim 193. 198. An isolated polypeptide comprising the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 199. The isolated polypeptide of claim 198, comprising the complete amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 200. The isolated polypeptide of claim 198, further comprising a heterologous polypeptide. 201. A composition comprising the polypeptide of claim 198. 202. An isolated polynucleotide comprising a nucleic acid encoding at least 50 contiguous amino acids of the amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 203. The isolated polynucleotide of claim 202, further comprising a heterologous polynucleotide. 204. A method of producing a recombinant vector comprising inserting the isolated polynucleotide of claim 202 into a vector. 205. A vector comprising the isolated polynucleotide of claim 202. 206. The vector of claim 205, wherein said polynucleotide is operably associated with a heterologous regulatory sequence. 207. A host cell comprising the isolated polynucleotide of claim 202. 208. The host cell of claim 207, wherein said isolated polynucleotide is operably associated with a heterologous regulatory sequence. 209. A method of producing a polypeptide encoded by the polynucleotide of claim 202, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide. 210. A polypeptide produced by the method of claim 209. 211. A composition comprising the polynucleotide of claim 202. 212. The complement of the isolated polynucleotide of claim 202. 213. An isolated polypeptide comprising 30 contiguous amino acids of the amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 214. The isolated polypeptide of claim 213, comprising 50 contiguous amino acids of the amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97641. 215. The isolated polypeptide of claim 213, further comprising a heterologous polypeptide. 216. A composition comprising the polypeptide of claim 213. |
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