| TECHNOLOGY |
NONVIRAL VECTORS are easier & less expensive to manufacture. The plasmids that are used in nonviral systems can be produced in bacteria such as E. coli. The same production facilities can be used to manufacture a variety of plasmids incorporating different genes. All other components of nonviral systems are chemical “so it’s like a real pharmaceutical” Co claims that it is able to condense plasmid DNA to “its theoretical minimum size” Most of their work is being done with POLYLYSINE deriv. They can compact a “average size” plasmid to 20-25 nm. Co believes that this small size allows the particles to get into the nucleus of nondividing cells, with efficiencies 3-4 orders of magnitude higher than those seen with noncompact ed DNA, through the nuclear pores, which are 25-50 nm wide. It is usually thought that DNA enters the nucleus during cell division, because the nuclear membrane breaks down during mitosisPoly(ethylene glycol) added to the particles helps keep them stable at high concn. “We use the polylysine because, when it’s complexed with DNA, it’s not toxic When you use a lipid-based system, often the lipids themselves are associated with some toxicity. “Polylysine by itself in large quantities can have some toxicity. But when polylysine, which is very positively charged, is complexed with DNA, which is very negatively charged, they combine, & all the toxicities seem to go away for both agents” Co is targeting the polymeric immunoglobulin receptor which is found in cells lining the airway |
| UPDATE | 11.01 |
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