| PATENT ASSIGNEE'S COUNTRY | USA |
| UPDATE | 09.99 |
| PATENT ASSIGNEE | Calgene |
| PATENT CLAIMS |
A method for obtaining an engineered plant acyl- ACP-ThioEsterase having an altered substrate specificity with respect to the acyl ACP substrates hydrolyzed by said ThioEsterase (A) Modifying a gene seq. encoding a first plant thioesterase protein to produce one or more modified thioesterase gene seq., wherein said modified seq. encode engineered acyl-ACP thioeste rases having substitutions, insertions or dele tionsof one or more aa. 230 to 285 of the consen sus numbering of thioesterase aa. seq. shown in FIG. 1 wherein said region corresponds tp residues 194 to 244 of SEQ ID NO:1, residues 194 to 244 of SEQ ID NO:2, residues 227 to 277 of SEQ ID NO:3, residues 221 to 271 of SEQ ID NO:4, residues 153 to 207 of SEQ ID NO:5 7 residues 170 to 224 of SEQ ID NO:6 (B) Expressing said modified gene seq. in host cell whereby said engineered plant thioesterases are produced (C) Assaying said engineered plant thioesterases to detect altered substrate specificity |
| PATENT PHOTOCOPY | Available on request |
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