PATENT CLAIMS |
In an immunoassay for DPD as analyte in fluid test medium in which a labeled Ab specific to DPD analyte is combined with the fluid test medium, which fluid test medium is then contacted with a solid support of nitrocellulose upon which the DPD analyte or deriv is immobilized so that labeled Ab which has not reacted with the DPD analyte in the test medium will react & be bound by the immobilized DPD analyte or deriv. thereof, the IMPROVEMENT which comprises immobilizing the DPD analyte or deriv on the solid support using a conjugate prepd by reacting DPD analyte or deriv with - Polyethylene glycol having MW = 6,000-50,000 functionalized on one or both ends with group reactive with DPD analyte or deriv which group is activated carboxy, amino, epoxy, halo, sulfhydryl, isocyanate maleimide or formyl & when functionalized on only one end with a reactive group, the PEG is functionalized on the other end with an unreactive capping group, wherein reactive group acts to bind the DPD analyte or deriv
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