Main > IMMUNOLOGY > Antibody > Monoclonal AntiBody (MAb) > Production > in Chicken

Product USA. A

PATENT ASSIGNEE'S COUNTRY USA
UPDATE 11.00
PATENT ASSIGNEE Arch Development
PATENT CLAIMS Production process
- Immunizing a chicken with an antigen compn
- Isolating B cells from said chicken
- Immortalizing said B cells
- Selecting an immortalized Ab producing B cell & prepg
nucleic acids encoding the antigen binding regions of the
light & heavy chain genes thereof
- Cloning said heavy & light chain antigen binding regions
resp., into vectors encoding (a) the constant & leader
regions of a mammalian immunoglobulin heavy chain (b)
the constant & leader regions of mammalian immunoglobu
lin light chain
- Transferring said vectors into suitable host cell
- Culturing said host cell
- Isolating said Ab
PATENT PHOTOCOPY Available on request

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