| PATENT ASSIGNEE'S COUNTRY | USA |
| UPDATE | 11.99 |
| PATENT ASSIGNEE | This data is not available for free |
| PATENT CLAIMS |
Method of continually growing human granulocytic Ehrlichia, E. equi, or E. phagocytophila comprising - Infecting a cell line, wherein the phenotype of the cell line is selected from the group consisting of a promelocytic leukemia cell line, an acute myelogenous leukemia cell li ne, a histiocytic lymphoma cell line, an acute monocytic leukemia cell line, & an embryonic lung cell line, with human granulocytic Ehrlichia, E. equi, or E. phagocylophila by co- culturing the cell line with blood cells infected with human granulocytic Ehrlichia, E. equi, or E. phagocytophila within suitable culture medium - Culturing the infected cell line with fresh uninfected cultured cells - Splitting the infected cell line in a split ratio of 1:2-1:3 of infected cells to additional said fresh uninfected cultured cells in order to maintain the culture - Identifying a cytopathic effect on the infected cell line, wherein the means for identifying the said effect comprise (i) Using an immunofluorescence assay, Western blot analysis, EIA or RIA to detect the presence of human granu locytic Ehrlichia, E. equi or E. phagocytophila using detec tor Ab specific for said human granulocytic Ehrlichia, E. equi or E. phagocytophila; (ii) Using PCR or RFLP to con firm the presence or absence of human granulocytic Ehrli chia, E. equi or E. phagocytophila in the cell lines with a primer or a 16S rDNA gene specific for said human granulo cytic Ehrlichia, E. equi or E. phagocytophila; or (iii) Obser ving degenerative cell morphology by light microscopy |
| PATENT PHOTOCOPY | Available on request |
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