| PATENT ASSIGNEE'S COUNTRY | USA |
| UPDATE | 09.99 |
| PATENT ASSIGNEE | American Home Products |
| PATENT CLAIMS |
Method for producing high yields of plasmid DNA in a culture of "E. coli" contg said plasmid DNA, comprising reducing the cell growth rate of said E. coli cells in culture by simultaneous feedback steps which maintain a desired pH & DOC, comprising - Monitoring the pH of culture & adding base to culture when said pH drops < first setpoint between 6.8-7.0 - Monitoring the pH of culture & adding nutrient medium to culture when said pH increases > second setpoint between 7.05-7.2 - Monitoring DOC of culture & increasing agitation speed in said culture when DOC decreases < first DOC setpoint between 10-50%, & maintaining said increased agitation speed once said first DOC setpoint is reached - Monitoring the DOC of culture & adding nutrient medium to culture when DOC increases > second DOC setpoint between 40-70% Wherein plasmid DNA production by said culture is maximized & protein & RNA production by culture is decreased |
| PATENT PHOTOCOPY | Available on request |
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