Main > PROTEINS > Interferon > IFN. Alpha > Mfg

Product Egypt. A

PATENT ASSIGNEE'S COUNTRY Egypt
UPDATE 12.00
PATENT ASSIGNEE This data is not available for free
PATENT CLAIMS Process for producing crude alpha-IFN
- Obtaining purified leukocyte from human blood
- Culturing said leukocytes in a medium in suspension culture
- Adjusting the T of the culture to 30-40C & pretreating the cul
ture by contacting the leukocytes with 10-1000 IU/ml alpha
IFN, beta-IFN or gamma-IFN & continuing the culturing of
leukocytes at 30-40C for 0.5-6 hours
- Adjusting the T of culture 35-39C & contacting the leukocyte
with first portion of 10-1000 haemagglutination units/ml of
Sendai virus & continuing culture for 0.5-3 hours
- Lowering T to < 30C & continuing culture for 6-36 hours
- Contact leukocyte with second portion of 10-1000 haema
gglutination units/ml of Sendai virus
- Separating the leukocytes from culture medium to obtain a
cell-free culture medium comprising crude alpha-IFN
PATENT PHOTOCOPY Available on request

Want more information ?
Interested in the hidden information ?
Click here and do your request.


back