PATENT ASSIGNEE'S COUNTRY | USA |
UPDATE | 12.00 |
PATENT ASSIGNEE | This data is not available for free |
PATENT CLAIMS |
A fluorescent immuno-bead non-agglutination sandwich assay kit for use in conjunction with flow cytometry for the simultaneous detection of sera of the antinuclear Ab to RNP (ribonucleoprotein) selected from group consisting of mixed connective disease, systemic lupus erythematosis (SLE), Sjogren's syndrome, scleroderma & polymyositis, Sm (Smith antigen) in SLE; SS-A in Sjogren's syndrome & SLE, SS-B in Sjogren's syndrome & SLE & Scl-70 in scleroderma, using a flow cytometer to analyze at least the size & the fluorescence of a plurality of single microsphere complexes & including a bead carrier, a bound antigen or analyte, a primary Ab against the bound antigen, a sec anti-species Ab attached to the primary Ab, & an indicator attached to the anti-species Ab comprising - 4 micro.m sized latex beads - 5 micro.m particle sized latex beads - 6 & 7 micro.m particle sized latex beads - 10 micro.m particle sized latex beads - Sm/RNP Complex antigen - Sm antigen - SS-A (Ro) antigen - SS-B (La) antigen - Scl-70 antigen - Lyophilized (L) Anti-RNP - L Anti-Sm - L Anti-SS-A (Ro) - L Anti-Sm - L Anti-SSB (La) - L Anti-Scl-70 - Goat anti-human IgG F(ab')2-[FITC] fluorescein isothiocyan late - Na carbonate - Na bicarbonate - Bovine Serum Albumin |
PATENT PHOTOCOPY | Available on request |
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