| PATENT ASSIGNEE'S COUNTRY | USA |
| UPDATE | 02.00 |
| PATENT ASSIGNEE | Alcon Labs |
| PATENT CLAIMS |
Endotoxin-induced inflammation assay for evaluating a drug - Adm a drug to ocular tissue of an experimental animal - Inducing inflammation in animal by adm endotoxin - Isolating the ocular tissue 16-30 hours after adm of endoto xin - Homogenizing the ocular tissue in a buffered compn com prising a non-interfering thiol reactive compd, compn with pH 5-8 - Centrifuging the compn to recover neutrophils (PMN) & myeloperoxidase (MPO) in a pellet - Solubilizing the said pellet in a buffered compn contg hexadecyltrimethylammonium bromide (HTA-Br) in order to release MPO from PMN azurophil granules, wherein the compn has pH 5-7 - Centrifuging compn to recover MPO in the supernatant - Prepg a buffered compn comprising said supernatant, o-dianisidine (o-DA), H2O2 & HTA-Br, compn has pH 6 - Determn MPO activity by spectrophotometrically monito ring at 460 nm change in absorbance of compn due to o-DA oxidation |
| PATENT PHOTOCOPY | Available on request |
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