| CLASSIFICATION | CommPat. Market |
| MARKETER'S COUNTRY | Netherlands |
| PATENT ASSIGNEE'S COUNTRY | Netherlands |
| PRODUCER'S COUNTRY | Netherlands |
| UPDATE | 05.00 |
| MARKETING BY | This data is not available for free |
| PATENT ASSIGNEE | This data is not available for free |
| PATENT CLAIMS |
A kit for the isothermal amplification of a specific nucleic acid seq or its complement according to a process for the amplification of an RNA first template or second DNA template as defined herein, at a rel. constant T & without serial addition of reagents - Providing a single reaction medium including first primer second primer having an anti-sense seq of a promoter, an RNA polymerase that recognizes said promoter, & a DNA polymerase, wherein the ribonuclease activity of said DNA polymerase hydrolyzes the RNA of an RNA-DNA hybrid without hydrolyzing single or double-stranded RNA or DNA - Providing in said single reaction medium said RNA first template, such that the following staps take place: (1) Hybri dizing said first primer to said RNA first template, (2) Synthe sizing from said DNA polymerase & said RNA first template a DNA second template by extending said first primer & thereby forming an RNA-DNA hybrid intermediate; (3) Hydro lyzing the RNA of said RNA-DNA hybrid intermediate; (4) Hybridizing said second primer to said DNA second templa te; (5) Synthesizing from said DNA polymerase, said se cond primer, & said DNA second template a functional pro moter recognized by an RNA polymerase. (6) Having said RNA polymerase recognizing said functional promoter & transcribing said DNA second template, thereby providing copies of said RNA first template - Maintaining said conditions for a time sufficient to achieve a desired amplification of said RNA first template or said DNA second template |
| PATENT PHOTOCOPY | Available on request |
| PRODUCTION BY | This data is not available for free |
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