| PATENT ASSIGNEE'S COUNTRY | Netherlands |
| UPDATE | 02.00 |
| PATENT ASSIGNEE | Akzo Nobel |
| PATENT CLAIMS |
- Injecting a mammal with a cell surface antigen-comprising material, wherein said material is selected from the group consisting of i) whole T cells & ii) a membrane fraction obtai ned by treating whole T cells - Isolating B-cells-contg cell fraction from the spleen of ma mmal - Enriching said cell fraction obtained in 2nd step in B-cells specific for said cell surface antigen by contacting the cell fraction with carrier-bound material of cells related to said whole cells, said related cells lacking said cell surface antigen, & separating B-cells bound to said carrier-bound material of related cells from the enriched unbound B-cells contg cell fraction to be used in the next step - Subjecting the enriched B-cells-contg cell fraction obtained in 3rd step to limiting dilution followed by clonal expansion - Selecting a B-cell clone & immortalizing said selected B-cell clone using a small-scale fusion technique - Selecting & cloning a hybridoma capable of producing Ab which specifically bind said cell surface antigen - Isolating a MAb-comprising fraction from supernatant of said hybridoma |
| PATENT PHOTOCOPY | Available on request |
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