| PATENT ASSIGNEE'S COUNTRY | Japan |
| UPDATE | 07.00 |
| PATENT ASSIGNEE | This data is not available for free |
| PATENT CLAIMS |
Into natural-type human activin A having a biological activity - Protecting thiol groups in solubilized denatured human acti vin A with all thiol groups free, with glutathione &/or Na sul fite, & (b1) dialyzing the protected denatured human activin A against a refolding buffer & then adding a thiol compd thereto, to exchange disulfide bonds in the denatured acti vin A & produce human activin A having a biological activi ty Wherein the refolding buffer contains contg taurodeoxycho lic acid Wherein the refolding buffer permits the structure of natural- type human activin A dissolved at concn of 0.1 mg/ml in the refolding buffer, when denatured by reduction with 0.2 mM dithiothreitol (DTT), to be maintained stably by > 20% than by a Tris buffer contg 1.5 M urea, 20 mM Tris-HCl, at pH 8.5 |
| PATENT PHOTOCOPY | Available on request |
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