Main > PROTEINS > Proteomics > Bacteria Proteomics > Bacillus subtilis > Transglutaminase

Product Japan. A. No. 1

PATENT ASSIGNEE'S COUNTRY Japan
UPDATE 09.99
PATENT ASSIGNEE This data is not available for free
PATENT CLAIMS A method for processing a protein, non-protein amino acid polymer or peptide having crosslinked structure
- Contacting glutamine & lysine residues in a protein etc.,
with a transglutaminase obtained from "Bacillus subtilis" to
form intermolecular or intramolecular, crosslinked epsilon-
(gamma-Glu)-Lys bonds between or in the molecules of the
protein etc
Wherein said transglutaminase has the following physicoche
mical properties
- It is active at pH = 7-9
- It is active at 40-65C
- It is stable < 60C
- The enzymatic activity is not dependent on Ca ion & has an
activity > 50% in presence of 5 mM of Ca ion
- Inhibited by N-Et maleimide, cystamine or NH4 sulfate
- Not inhibited by EDTA, dithiothreitol or HS-CH2-OH
- Has MW of (i) 18,000-22,000 as measured by gel permea
tion, & (ii) 28,000-30,000 measured by SDS-PAGE
- Catalyzes the transacylation of the gamma-carboxyamide
group in glutamine residue(s) in peptide chain
PATENT PHOTOCOPY Available on request

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