PATENT ASSIGNEE'S COUNTRY | USA |
UPDATE | 10.00 |
PATENT ASSIGNEE | Agilent Technologies |
PATENT CLAIMS |
Method for producing linearly amplified amounts of anti-sen se RNA from mRNA, said method comprising - Converting mRNA to double-stranded cDNA, wherein one terminus of said double-stranded cDNA comprises an RNA polymerase promoter region - Transcribing said double-stranded cDNA with an RNA polymerase & ribonucleotides into antisense RNA in the presence of a reverse transcriptase that is incapable of RNA-dependent DNA polymerase activity during said transcribing step |
PATENT PHOTOCOPY | Available on request |
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