Main > NUCLEIC ACID > RNA > mRNA > Amplification > Linear Amplification

Product USA. A

PATENT ASSIGNEE'S COUNTRY USA
UPDATE 10.00
PATENT ASSIGNEE Agilent Technologies
PATENT CLAIMS Method for producing linearly amplified amounts of anti-sen
se RNA from mRNA, said method comprising
- Converting mRNA to double-stranded cDNA, wherein one
terminus of said double-stranded cDNA comprises an RNA
polymerase promoter region
- Transcribing said double-stranded cDNA with an RNA
polymerase & ribonucleotides into antisense RNA in the
presence of a reverse transcriptase that is incapable of
RNA-dependent DNA polymerase activity during said
transcribing step
PATENT PHOTOCOPY Available on request

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