| PATENT ASSIGNEE'S COUNTRY | USA |
| UPDATE | 06.00 |
| PATENT ASSIGNEE | This data is not available for free |
| PATENT CLAIMS |
Method for nucleic acid sample clean-up using a substrate having a surface & an enrichment microchannel, a main electrophoretic flowpath & discharge outlet, formed in the substrate, said enrichment microchannel comprising an enrichment region & said main electrophoretic flowpath comprising a working region, said enrichment microchannel being in fluid transfer relationship with said main electrophoretic flowpath, & an affinity binding pair having complementary first & second binding members, said first binding member comprising a label, said method comprising the steps of: - Introducing a nucleic acid mixt having nucleic acid portion & a waste portion into the enrichment microchannel - Combining the first binding member of the affinity binding pair with the nucleic acid portion of at least some of the nu cleic acid mixt to form a labeled nucleic acid portion of bound entities between nucleic acid mole. of said nucleic acid portion & said labeled first binding member - Contacting in the enrichment region bound entities with the second binding member of the affinity binding pair, which is bound to at least one solid support to capture at least a part of the bound entities to form captured nucleic acid por tion - Washing the captured nucleic acid portion to direct the waste portion & the nucleic acid portion excluding the captu red nucleic acid portion through said discharge outlet & away from the working region - Releasing the captured nucleic acid portion by competitive displacement of the first binding member bound to the captu red nucleic acid portion with a competitive displacing mem ber having a higher affinity for the second binding member than the first binding member to yield a purified nucleic acid portion - Transporting the purified nucleic acid portion to the wor king region - Whereby the purified nucleic acid portion is processed or analyzed or processed & analyzed in the working region |
| PATENT PHOTOCOPY | Available on request |
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