PATENT ASSIGNEE'S COUNTRY | USA |
UPDATE | 09.00 |
PATENT ASSIGNEE | This data is not available for free |
PATENT CLAIMS |
Method for isolating recombinant beta-casein from prokaryo tic cells genetically engineered to produce beta-casein - Obtaining a crude prepn of said cells - Mixing said prepn with first soln comprising a buffer system, wherein said beta-casein is insoluble in first soln - Centrifuging said first suspension to obtain first pellet & first supernatant, wherein said first pellet includes all of said beta-casein - Suspending first pellet in second soln comprising a buffer system & protein solubilizing agent, wherein said beta-ca sein is soluble in second soln - Centrifuging second suspension to obtain second pellet & second supernatant, wherein second supernatant inclu des all of said beta-casein |
PATENT PHOTOCOPY | Available on request |
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