Main > PROTEINS > Casein > Beta Casein > Recombinant Production

Product USA. A. No. 3

PATENT ASSIGNEE'S COUNTRY USA
UPDATE 09.00
PATENT ASSIGNEE This data is not available for free
PATENT CLAIMS Method for isolating recombinant beta-casein from prokaryo
tic cells genetically engineered to produce beta-casein
- Obtaining a crude prepn of said cells
- Mixing said prepn with first soln comprising a buffer system,
wherein said beta-casein is insoluble in first soln
- Centrifuging said first suspension to obtain first pellet &
first supernatant, wherein said first pellet includes all of said
beta-casein
- Suspending first pellet in second soln comprising a buffer
system & protein solubilizing agent, wherein said beta-ca
sein is soluble in second soln
- Centrifuging second suspension to obtain second pellet
& second supernatant, wherein second supernatant inclu
des all of said beta-casein
PATENT PHOTOCOPY Available on request

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