| CORP. SALE | BMC = Cyclohexane-1,2-Di(NH-CO-CH2-SH) |
| TECHNOLOGY |
Proteins with > 2 disulfide bonds couldn't be expressed in active form in any significant amounts. Instead they end up as insoluble MISFOLDED proteins The present approach; incorrectly folded proteins are REFOLDED by small-mole. enzyme mimic. When multiple disulfide contg proteins are expressed in yeast, many form nonnative disulfide bonds that prevent them from folding properly. Disulfide cleavage & re-formation is catalyzed in cells by PDI enzyme The researchers synthesized BMC (trans-1,2-bis(mercapto- acetamido)cyclohexane (see structure) & tested. They found that it catalyzes disulfide bond isomerization both in vitro & in vivo Examples - BMC catalyzes the in vitro formation of native ribonuclease A from a disulfide-scrambled version of the enzyme - Increases yields of a native acid phosphatase with 8 disul fide bonds in a yeast expression system by factor of 3 |
| UPDATE | 12.99 |
| AUTHOR | This data is not available for free |
| LITERATURE REF. | This data is not available for free |
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