Main > INFECTIOUS DISEASES > Dengue > Virus > Infection > Diagnostics

Product USA. U

PATENT ASSIGNEE'S COUNTRY USA
UPDATE 08.99
PATENT ASSIGNEE University of Massachusetts at Worcester
PATENT CLAIMS Method of detecting dengue virus & not Japanese encephalitis virus in sample
- Incubating RNA extracted from sample with reverse trans
criptase & first dengue virus primer including > 15 consecu
tive nucleotides of SEQ ID NO:3 wherein said primer is com
plementary yo portion of the 3' noncoding region in dengue
virus nucleic acid for time to allow double stranded nucleic
acid to form
- Adding second primer including > 15 nucleotides of SEQ
ID NO:1 identical to 3' region in virus & thermostable DNA
polymerase
- Incubating double stranded acid for amplification
- Detecting reaction products
PATENT PHOTOCOPY Available on request

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